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The in vitro effects of selected isoflavonoids on isolated rat aorta
Novýsedláková, Alena ; Pourová, Jana (advisor) ; Vopršalová, Marie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Alena Novýsedláková Supervisor: PharmDr. Jana Pourová, PhD. Title of Thesis: In vitro effects od selected isoflavonoids on isolated rat aorta Abstract: The aim of this study was to determine whether the selected isoflavonoids are able to vasodilate aorta, what structural features might be responsible for a relaxing activity and estimate the mechanism of action. The experiment we carried out the classical in vitro method on the isolated rat aorta, and the results evaluated by the computer program GraphPad. Only glycitin did not show statistically significant relaxation of blood vessels, which can be attributed to ineffective glycosidic form. Other izoflavonoids- glycitein, daidzein and tectorigenin seem to be promising in terms of potential vasodilatation. Further studies would be needed to verify also the in vivo effects. Likely structural features include the hydroxy group at 7-position and a methoxy group at position 8 of the core od isoflavonoids. The mechanism of action is not yet known, but most likely appears endothelium-dependent mechanisms, alpha 1 receptor antagonism and agonism of muscarinic M3 receptor. It may be also involved in the effect of the antioxidant and...
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Metabolism of isoflavonoids in human organism
Šramarová, Pavlína ; Karlíčková, Jana (advisor) ; Jahodář, Luděk (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Bc. Pavlína Šramarová Diploma thesis supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of thesis 2015/2016: Metabolism of isoflavonoids in human organism, pp. 54 Isoflavonoids are subgroup of flavonoids, belonging to the phytoestrogenic compounds. These compounds occur especially in leguminous, mainly in soya (Glycine max (L.) Merr). They have a positive impact on human health, especially for its estrogenic effects, due to which are applied in the fight against osteoporosis, cardiovascular diseases, atherosclerosis and for moderation of menopausal symptoms. Furthermore they exhibit antibacterial activity and protect cells against damage of DNA. This thesis deals with their metabolism and action in human and animal organism, also with the influence of intestinal microflora to transform these isoflavonoids into metabolites, as well as the effects of these metabolites. There are also mentioned these isoflavonoids: daidzein, genistein, biochanin A, formononetin, calycosin, prunetin, puerarin, cladrin, tectorigenin and kakkalid. Key words: isoflavonoids, metabolism, human organism
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Secondary metabolites of plant tissue culture of Trifolium pratense L.
Novotná, Hana ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Hana Novotná Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of diploma thesis: Secondary metabolites of plant tissue culture of Trifolium pratense L. Explant cultures are perspective sources of secondary metabolites. Nevertheless production of flavonoids and isoflavonoids by the suspension culture of Trifolium pratense L. is not high. Elicitation is one of the methods used to enhance the biosynthesis of secondary metabolites. Elicitation induces physiological changes, stimulates defensive or stress-induced reactions in plants and subsequently triggers the synthesis of secondary metabolites. The objective of this study was to observe the influence of two elicitors - abscisic acid and ascorbic acid - on the production of flavonoids and isoflavonoids by the Trifolium pratense L. suspension culture (Sprint variety). The culture was cultivated in Gamborg medium to which 2 mg.l-1 of 2,4- dichlorophenoxyacetic acid and 2 mg.l-1 of 6-benzylaminopurine were added, at the temperature of 25 řC and 16 hours light / 8 hours dark period. The best elicitation effect of abscisic acid on the production of flavonoids and isoflavonoids was observed after a 6-hour application of the highest 500 µmol.l-1...
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The study of secondary metabolites in plant tissue cultures I
Blahušová, Adriana ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Adriana Blahušová Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of diploma thesis: The study of secondary metabolites in plant tissue cultures I A principal precondition for successful elicitation used to increase the production of secondary metabolites is, among other, finding a suitable elicitor, its concentration and the optimal period of time of the action of the elicitor on the plant culture in vitro, which was the aim of the present diploma thesis. The effect was examined of a 6, 24, 48 and 168 hour action of the solution of chitosan (in four concentrations) on the production of flavonoids in the suspension culture Trifolium pratense L. (variety Sprint, Tempus and DO-8). The culture was cultivated in Gamborg medium to which 2 mg/l of 2,4- dichlorophenoxyacetic acid and 2 mg/l of 6-benzylaminopurine were added, at the temperature of 25řC and 16 hours light/8 hours dark period. The best elicitation effect of chitosan on the production of flavonoids was the lowest concentration of 1 mg/100ml in all three studied varieties after 48 hours of application.
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The study of secondary metabolites in plant tissue cultures I
Blahušová, Adriana ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Adriana Blahušová Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of diploma thesis: The study of secondary metabolites in plant tissue cultures I A principal precondition for successful elicitation used to increase the production of secondary metabolites is, among other, finding a suitable elicitor, its concentration and the optimal period of time of the action of the elicitor on the plant culture in vitro, which was the aim of the present diploma thesis. The effect was examined of a 6, 24, 48 and 168 hour action of the solution of chitosan (in four concentrations) on the production of flavonoids in the suspension culture Trifolium pratense L. (variety Sprint, Tempus and DO-8). The culture was cultivated in Gamborg medium to which 2 mg/l of 2,4- dichlorophenoxyacetic acid and 2 mg/l of 6-benzylaminopurine were added, at the temperature of 25řC and 16 hours light/8 hours dark period. The best elicitation effect of chitosan on the production of flavonoids was the lowest concentration of 1 mg/100ml in all three studied varieties after 48 hours of application.
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The study of secondary metabolites in plant tissue cultures I
Blahušová, Adriana ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Adriana Blahušová Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of diploma thesis: The study of secondary metabolites in plant tissue cultures I A principal precondition for successful elicitation used to increase the production of secondary metabolites is, among other, finding a suitable elicitor, its concentration and the optimal period of time of the action of the elicitor on the plant culture in vitro, which was the aim of the present diploma thesis. The effect was examined of a 6, 24, 48 and 168 hour action of the solution of chitosan (in four concentrations) on the production of flavonoids in the suspension culture Trifolium pratense L. (variety Sprint, Tempus and DO-8). The culture was cultivated in Gamborg medium to which 2 mg/l of 2,4- dichlorophenoxyacetic acid and 2 mg/l of 6-benzylaminopurine were added, at the temperature of 25řC and 16 hours light/8 hours dark period. The best elicitation effect of chitosan on the production of flavonoids was the lowest concentration of 1 mg/100ml in all three studied varieties after 48 hours of application.
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The effect of methylviologen on secondary metabolites production in in vitro culture of Genista tinctoria
Macová, Alena ; Tůmová, Lenka (advisor) ; Kašparová, Marie (referee)
Elicitation is one of possible methods for increasing the secondary metabolites production in plant cell cultures. This paper explores the potential effect of methylviologene on isoflavonoids production in Genista tinctoria L. suspension and callus cultures. Schenk and Hildebrandt nutrient medium was used with the addition of growth regulators 2,4- D in concentration of 5 mg/L and kinetin of 1 mg /L of medium. Elicitor was added in the form of ethanol solution at concentration of 2.19·10-2 mol l-1 , 2.19·10-3 mol l-1 , 2.19·10-4 mol l-1 . Samples were taken after 6, 12, 24, 48, 72 and 168 hours of elicitor exposure. Control samples were collected at 6, 24, 72 and 168 hours. The isoflavonoid content in suspension and callus cultures and in nutrient medium was evaluated by high performance liquid chromatography. The biggest increase of isoflavonoid content in callus culture was reached in daidzein content after 48 hours elicitor treatment in concentration of 2.19·10-3 mol l-1 (8.5 mg/g DW) and 2.19·10-4 mol l-1 (1.6 mg/g DW). The production of genistin, genistein and formononetin was slightly increased or zero compared to controls. Biochanin A was almost absent in the samples. The highest level of isoflavonoids was measured in the suspension culture after 48 hours elicitor application in the...
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Cardiovascular effects of isoflavonoids
Jančíková, Lenka ; Pourová, Jana (advisor) ; Vopršalová, Marie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Bc. Lenka Jančíková Supervisor: PharmDr. Jana Pourová, Ph.D. Title of diploma thesis: Cardiovascular effects of isoflavonoids Background: The aim of this thesis is to summarize existing findings about natural vegetable substances known as phytoestrogens-isoflavones and to map the results of latest studies focused on cardiovascular effects of isoflavones. Main findings: Available data suggest that isoflavones and their metabolites have positive effect on human organism. These include antiaterosclerotic, antimenopausal and anticarcinogenic effects and a positive effect on osteoporosis. Positive effect on cardiovascular system includes antihypertensive, anti-inflammatory, anti-angiogenic, antiproliferative and antiaggregating effects. On the other hand, there are studies that point out possible risks related to mainly long-term use of isoflavones. Nevertheless, even though the findings of cardiovascular effects are not always consistent, most of them confirm positive effects of isoflavones on cardiovascular system. Conclusion: Isoflavones and their metabolites are a very interesting group of substances with natural origin and carry a potential for possible development of new drugs. Therefore, they...
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Knowledge of less common isoflavonoids in plants
Cymbál, Martin ; Karlíčková, Jana (advisor) ; Jahodář, Luděk (referee)
Charles University Pharmaceutical Faculty in Hradec Králové Department of Pharmaceutical Botany Candidate: Martin Cymbál, DiS. Supervisor: PharmDr. Jana Karlíčková, Ph.D. Bachelor thesis 2016/2017: Knowledge of less common isoflavonoids in plants, pp. 78. Isoflavonoids are a group of 3-phenylchromen-4-one phenol flavonoids, many of which are biologically active. Isoflavonoids and their derivatives are sometimes referred to as phytoestrogens, because their structure and effect are similar to steroid hormones and are fed to estrogen receptors in the body. Isoflavonoids play an important role in human food as a health promoting plant substance. Isoflavonoids and their related compounds are used in many dietary supplements and only in one registered phytopharmac (Menofem). Recently, some natural isoflavones have been identified as toxins, eg. biliatrasone, which can cause biliary atresia if infants are exposed to this substance. However, with years of research and finding of new isoflavonoids together with understanding of their mechanism of action and their structure they have gained importance. Over the last ten years isoflavonoids have been subject to extensive research and analysis mainly because of their countless effects that were confirmed by studies. Many other in vitro and in vivo experiments need to be...
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The in vitro effects of selected isoflavonoids on isolated rat aorta
Novýsedláková, Alena ; Pourová, Jana (advisor) ; Vopršalová, Marie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Alena Novýsedláková Supervisor: PharmDr. Jana Pourová, PhD. Title of Thesis: In vitro effects od selected isoflavonoids on isolated rat aorta Abstract: The aim of this study was to determine whether the selected isoflavonoids are able to vasodilate aorta, what structural features might be responsible for a relaxing activity and estimate the mechanism of action. The experiment we carried out the classical in vitro method on the isolated rat aorta, and the results evaluated by the computer program GraphPad. Only glycitin did not show statistically significant relaxation of blood vessels, which can be attributed to ineffective glycosidic form. Other izoflavonoids- glycitein, daidzein and tectorigenin seem to be promising in terms of potential vasodilatation. Further studies would be needed to verify also the in vivo effects. Likely structural features include the hydroxy group at 7-position and a methoxy group at position 8 of the core od isoflavonoids. The mechanism of action is not yet known, but most likely appears endothelium-dependent mechanisms, alpha 1 receptor antagonism and agonism of muscarinic M3 receptor. It may be also involved in the effect of the antioxidant and...
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